The mouse monoclonal antibody clone HI186 binds to human CD52, a 25-29 kDa glycosylphosphatidylinositol (GPI)-anchored glycoprotein. CD52, also known as Cambridge pathology 1 antigen (CAMPATH-1), is expressed in high levels on mature lymphocytes and the surface of thymocytes. It is also present in NK cells, macrophages/monocytes, eosinophils, neutrophils, and dendritic cells. It is absent from plasma cells, platelets, and erythrocytes. CD52 is known to be efficient in complement-mediated lysis and antibody-mediated cellular toxicity. It is clinically used as a drug target for various diseases, including lymphoma, transplant rejection, and autoimmune disease.

PRODUCT DETAILS

Clone: HI186

Specificity: CD52

Reactivity: Human, Rhesus Macaque, Cynomolgus Monkey

Format: APC

Isotype: Mouse IgG2b

Tested Dilution: 5 µL/Test

Application: FC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Flow cytometry: The suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application. The content of a vial (0.5 mL) is sufficient for 100 tests.

For research use only. Not intended for use in diagnostic procedures.