The mouse monoclonal antibody clone BNI3 binds to human CD152, commonly known as CTLA-4, a 33-37 kDa protein expressed as a homodimer on the surface of activated T and B cells, and on thymocytes. CTLA-4 is structurally similar to the T cell co-stimulatory molecule CD28, yet functionally disparate. Both CTLA-4 and CD28 interact with the co-stimulatory molecules CD80 (B7-1) and CD86 (B7-2) on antigen-presenting cells, with CTLA-4 displaying a higher avidity than CD28. While CD28 typically delivers a potent co-stimulatory signal in support of T cell activation, CTLA-4 appears to act as a negative regulator of T cell activation and may contribute to the suppressor function of Treg cells. CTLA-4 proteins may be initially sequestered within Golgi vesicles, from which they can be transferred to and from the cell surface, a mechanism by which Treg cells can selectively impart suppressive functions.

PRODUCT DETAILS

Clone: BNI3

Specificity: CD152 (CTLA-4)

Reactivity: Human, Baboon, Cynomolgus Monkey, Rhesus Macaque

Format: Alexa Fluor™ 647

Isotype: Mouse IgG2a, k

Tested Dilution: 5 µL/Test

Application: FC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Flow cytometry: The suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application. The content of a vial (0.5 mL) is sufficient for 100 tests.

REFERENCES

1. Castan J, Tenner-Racz K, et al. 1997. Immunology. 90:265-271.
2. Zola H. 2007. Leukocyte and stromal cell molecules: the CD markers. Hoboken, N.J.: Wiley-Liss.

For research use only. Not intended for use in diagnostic procedures.