PRODUCT DETAILS

Host: Rat

Isotype: IgG2c, kappa

Clonality: Monoclonal

Clone: 5H12

Format: Alexa Fluor 488

Reactivity: Ms

Application: Flow Cytometry

Tested Dilution: Assay-Dependent

Concentration: 0.5 mg/mL

Storage: 4°C, store in dark, DO NOT FREEZE!

Formulation: PBS with 0.09% sodium azide; pH 7.2

Purification: Affinity chromatography

Data Sheet: TDS

Specific Information

Description: This 5H12 monoclonal antibody reacts with mouse Autoimmune Regulator (AIRE). This 58-kDa transcription factor is highly expressed within the perinuclear region of mature medullary thymic epithelial cells (mTECs). However, expression of AIRE protein in the periphery remains unclear despite the detection of AIRE mRNA in the lymph node, lymphoid stromal cells, monocytes, and dendritic cells. Studies demonstrate that AIRE is involved in thymocyte development, in particular the regulation of tissue-specific antigen expression during negative selection. Other biological functions for AIRE include chemokine expression and antigen presentation.

The 5H12 antibody recognizes mouse AIRE isoforms 1 and 2.

Applications Reported: This 5H12 antibody has been reported for use in flow cytometric analysis and immunohistochemical staining of frozen tissue sections (IHC-F).

Applications Tested: This 5H12 antibody has been tested by immunohistochemistry on fixed, frozen mouse thymus at less than or equal to 10 µg/mL. It is recommended that this antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

 

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.