Cytek developed a 45-color panel that allows for comprehensive characterization of major cell lineages present in circulation including T cells, gamma delta T cells, NKT-like cells, B cells, NK cells, monocytes, basophils, dendritic cells, and ILCs.

The panel also supports outstanding discernment of cell activation, exhaustion, memory, and differentiation states of CD4+ and CD8+ T cells enabling an in-depth description of very distinct phenotypes associated with the complexity of the T cell memory response. 

To support this 45-color immunoprofiling panel, Cytek released a 6-color cFluor^® reagents bundle that can be used with reagents from other suppliers to stain for the full panel. 

This panel offers the unique opportunity to fully characterize, with high resolution, immunological profiles present in peripheral blood in the context of infectious diseases, autoimmunity, neurodegeneration, immunotherapy, and biomarker discovery.

Read: 45-Color Full Spectrum Flow Cytometry Panel for Deep Immunophenotyping of the Major Lineages Present in Human Peripheral Blood Mononuclear Cells with Emphasis on the T cell Memory Compartment

PRODUCT DETAILS

Tested Dilution: 5 μL / test

Application: Flow Cytometry

Storage: 2-8°C and protected from light.Do not freeze

Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (Origin USA)

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Human peripheral mononuclear cells (PBMC) have been tested to validate the performance of the 45-color panel. Please refer to the publication for the staining protocol.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

REFERENCES

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3. Mechtersheimer G, et al. 1991. Cancer Res. 51(4):1300-7
4. Stelin, S. et al. 2009. J Indian Soc Periodontol 13, 150-4
5. Künemund V, et al. J Cell Biol. 1988.106(1):213-23
6. Van Acker HH, et al. 2017. Front Immunol. 8:892
7. Crossland DL, et al. 2018. Oncogene. 37(27):3686-3697
8. Seidenfaden R, et al. 2006. Neurochem Int. 49(1):1-11

For Research Use Only. Not intended for use in diagnostic procedures.