cFluor® UV440 Anti-Human CD8a (RPA-T8)
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Description
The mouse monoclonal antibody clone RPA-T8 binds to the 32-34 kDa alpha chain of human CD8, known as CD8a or CD8 alpha. CD8a can form a homodimer (CD8 alpha-alpha) but is more commonly expressed as a heterodimer with a second chain known as CD8b or CD8 beta. CD8 acts as a co-receptor for antigen recognition and subsequent T cell activation that is initiated upon binding of the T cell receptor (TCR) to antigen-bearing MHC Class I molecules. The cytoplasmic domains of CD8 provide binding sites for the tyrosine kinase lck, facilitating intracellular signaling events that lead to T cell activation, development, and cytotoxic effector functions. CD8+ cytotoxic T cells (CTLs) play an important role in inducing cell death of tumor cells, as well as cells infected by viruses, bacteria, or parasites. If used together with an alternative Anti-Human CD8a clone, Hit8a, the RPA-T8 antibody will not block the binding of Hit8a to CD8a.
PRODUCT DETAILS
Clone: RPA-T8
Specificity: CD8a
Reactivity: Human
Format: cFluor® UV440*
Isotype: Mouse IgG1, k
Test Dilution: 5 µl/Test
Application: Flow Cytometry
Storage: 2-8°C protected from light. Do not freeze
Formulation: Phosphate-buffered saline, pH 7.2, containing 0.1% PEG 3350, 0.1% Pluronic F-68, 0.05% Tween-20, 0.09% sodium azide and 1% BSA (BSA Country of Origin USA)
RECOMMENDED USAGE
Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 μL per 1 million cells in a staining volume of 100 μL. If whole blood is analyzed, then use 5 μL per 100 μL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.
Please briefly centrifuge the reagent vial before use.
Use appropriate personal protective equipment per the product safety data sheet when using this product.
REFERENCES
Evans RL, et al. 1981. Immunol. 78:544
Muench M, et al. 1997. Blood 89:1364
Arno A et al. 1999. J. Infect. Dis. 180:56
For Research Use Only. Not intended for use in diagnostic procedures.
*Cytek® cFluor® UV440 is StarBright™ UltraViolet 445 Dye, manufactured and provided by Bio-Rad, Inc.
StarBright is a trademark of Bio-Rad Laboratories, Inc. in certain jurisdictions. StarBright Dyes are manufactured and provided by Bio-Rad under an Agreement between Bio-Rad supplied to Cytek, being subject to certain conditions.