violetFluor™ 450 Anti-Mouse CD8a (53-6.7)
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Description
The 53-6.7 antibody reacts with the 32-34 kDa alpha subunit of mouse CD8, known as CD8a or CD8 alpha. CD8a can form a homodimer (CD8 alpha-alpha), but is more commonly expressed as a heterodimer with a second chain known as CD8b or CD8 beta. CD8 acts as a co-receptor in antigen recognition and subsequent T cell activation that is initiated upon binding of the T cell receptor (TCR) to antigen-bearing MHC Class I molecules. The cytoplasmic domains of CD8 provide binding sites for the tyrosine kinase lck, facilitating intracellular signaling events that lead to T cell activation, development, and cytotoxic effector functions. CD8+ cytotoxic T cells (CTLs) play an important role in inducing cell death of tumor cells, as well as cells infected by virus, bacteria or parasites.
The 53-6.7 antibody is widely used as a phenotypic marker for mouse CD8a expression on cytotoxic T cells, thymocytes, as well as on certain cell types that do not also express the TCR, including some NK cells and lymphoid dendritic cells.
Recent Publications:
Wu M, Xia X, Hu J, Fowlkes NW, Li S. WSX1 act as a tumor suppressor in hepatocellular carcinoma by downregulating neoplastic PD-L1 expression. Nat Commun. 2021 Jun 9;12(1):3500. doi: 10.1038/s41467-021-23864-9. PMID: 34108491; PMCID: PMC8190270.
» View on Nature Communications
Uzodinma U. Uche, Ann R. Piccirillo, Shunsuke Kataoka, Stephanie J. Grebinoski, Louise M. D’Cruz, and Lawrence P. Kane. PIK3IP1/TrIP restricts activation of T cells through inhibition of PI3K/Akt. J Exp Med. 2018 Dec 3;215(12):3165-3179. doi: 10.1084/jem.20172018
» View on PubMed
Avery L, Filderman J, Szymczak-Workman AL, Kane LP4. Tim-3 co-stimulation promotes short-lived effector T cells, restricts memory precursors, and is dispensable for T cell exhaustion. Proc Natl Acad Sci U S A. 2018 Mar 6;115(10):2455-2460. doi: 10.1073/pnas.1712107115
Kersh AE, Edwards LJ and Evavold BD. 2014. J. Immunol. September 29, 2014, doi:10.4049/jimmunol.1401456 [Epub ahead of print] (Flow Cytometry)
Finkin S, Yuan D, Stein I, Taniguchi K, Weber A, Unger K, Browning JL, Goossens N, Nakagawa S, Gunasekara G, Schwartz ME et al. 2015. Nat Immunol. doi: 10.1038/ni.3290. (Flow Cytometry)
Name | violetFluor™ 450 Anti-Mouse CD8a (53-6.7) |
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Cat. No. | 75-0081 |
Alternative Names | CD8 alpha, Ly-2, Ly-35, Ly-B, Lyt-2 |
Gene ID | 12525 |
Clone | 53-6.7 |
Isotype | Rat IgG2a, κ |
Reactivity | Mouse |
Format | violetFluor™ 450 |
Application | Flow Cytometry |