The 2H7 monoclonal antibody binds to human CD20, a 33-37 kDa, four-transmembrane phosphoprotein. CD20 is present on all stages of B cell development except for early pro-B cells or plasma cells to play a role in B-cell activation, proliferation and differentiation¹. In some studies, CD20 is thought to act as a Ca2+ channel in the plasma membrane². It is also present on a T cell subset at low levels as well as some follicular dendritic cells.

The antibody was conjugated to a fluorophore and purified by affinity chromatography.

PRODUCT DETAILS

Clone: 2H7

Specificity: CD20

Reactivity: Human, Baboon, Capuchin Monkey, Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus, Squirrel Monkey

Format: cFluor^® V610

Isotype: Mouse IgG2b, κ

Tested Dilution: 5 µL / test

Application: Flow Cytometry

Storage: 2-8°C and protected from light. Do not freeze

Formulation: Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (BSA Country of Origin USA)

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

REFERENCES

1. Tedder TF, et al. 1994. Immunol Today. 15(9):450–4
2. Putney JW Jr. 1999. Proc Natl Acad Sci U S A. 96(26):14669-14671

For research use only. Not intended for use in diagnostic procedures.