cFluor® V435 Anti-Human CD4 (SK3)
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Description
The SK3 monoclonal antibody binds to human CD4, a 59-kDa type I transmembrane glycoprotein in the immunoglobulin superfamily. The CD4 molecule is expressed predominantly on thymocytes and a subpopulation of mature T-helper lymphocytes. It is also present on monocytes at low levels1,3. CD4 plays a role in cell-cell interaction by acting as a co-receptor for MHC class II in antigen recognition. CD4 also has been shown to bind glycoprotein 120 on external envelope of HIV2.
The antibody was purified by affinity chromatography and conjugated with a fluorophore under optimal conditions.
PRODUCT DETAILS
Clone: SK3
Specificity: CD4
Reactivity: Human
Format: cFluor® V435*
Isotype: Mouse IgG1, κ
Tested Dilution: 5 µL / test
Application: Flow Cytometry
Storage: 2-8°C and protected from light. Do not freeze
Formulation: Phosphate-buffered saline, pH 7.2, containing 0.1% PEG 3350, 0.1% Pluronic F-68, 0.05% Tween-20, 0.09% sodium azide and 1% BSA (BSA Country of Origin USA)
RECOMMENDED USAGE
Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.
Please briefly centrifuge the reagent vial before use.
Use appropriate personal protective equipment per the product safety data sheet when using this product.
REFERENCES
- Evans RL, et al. 1981. Immunol. 78:544
- Arno A et al. 1999. J. Infect. Dis. 180:56
- Muench M, et al. 1997. Blood 89:1364
For research use only. Not intended for use in diagnostic procedures.
*Cytek® cFluor® V435 is StarBright™ Violet 440 Dye, manufactured and provided by Bio-Rad, Inc.
StarBright is a trademark of Bio-Rad Laboratories, Inc. in certain jurisdictions. StarBright Dyes are manufactured and provided by BioRad under an Agreement between Bio-Rad supplied to a company, being subject to certain conditions.