cFluor® V435 Anti-Human CD14 (MEM-18)
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Description
The MEM-18 monoclonal antibody binds to human CD14, a 53-55-kDa glycosylphosphatidylinositol (GPI)- linked membrane glycoprotein that works as a receptor on myeloid cells for ligands such as lipopolysaccharide (LPS)1. It is found that CD14 is a receptor for and binds to complexes of LPS and LBP with high affinity2. It expresses on monocytes and macrophages at high levels. It is also present in some interfollicular dendritic cells and macrophages, reticular dendritic cells and Langerhans cells.
The antibody was purified by affinity chromatography and conjugated with a fluorophore under optimal conditions.
PRODUCT DETAILS
Clone: MEM-18
Specificity: CD14
Reactivity: Human
Format: cFluor® V435*
Isotype: Mouse IgG1
Tested Dilution: 5 µL / test
Application: Flow Cytometry
Storage: 2-8°C and protected from light. Do not freeze
Formulation: Phosphate-buffered saline, pH 7.2, containing 0.1% PEG 3350, 0.1% Pluronic F-68, 0.05% Tween-20, 0.09% sodium azide and 1% BSA (BSA Country of Origin USA)
RECOMMENDED USAGE
Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.
Please briefly centrifuge the reagent vial before use.
Use appropriate personal protective equipment per the product safety data sheet when using this product.
REFERENCES
- Pugin J, et al. 1998. Infect Immun. 66:1174
- Wright SD, et al. 1990. Science. 249:1431
For research use only. Not intended for use in diagnostic procedures.
*Cytek® cFluor® V435 is StarBright™ Violet 440 Dye, manufactured and provided by Bio-Rad, Inc.
StarBright is a trademark of Bio-Rad Laboratories, Inc. in certain jurisdictions. StarBright Dyes are manufactured and provided by BioRad under an Agreement between Bio-Rad supplied to a company, being subject to certain conditions.