The mouse monoclonal antibody clone 2D1 binds to human CD45. Human CD45, also known as Leukocyte Common Antigen (LCA), is one of the most abundant hematopoietic markers. It is a 180-220 kDa single-chain type I transmembrane protein expressed at high levels on all cells of hematopoietic origin, except erythrocytes, platelets, and non-hematopoietic cells. Specifically, CD45 is found on the surface of T and B lymphocytes, natural killer cells, monocytes, macrophages, dendritic cells, neutrophils, eosinophils, and basophils. CD45 functions as a protein tyrosine phosphatase with several isoforms, each generated and expressed in cell-specific patterns. With its broad cell distribution, CD45 plays a critical role in many leukocyte functions by regulating signal transduction and cell activation associated with the T cell receptor, B cell receptor, and IL-2 receptor. Other forms of CD45 with more restricted cellular expression include CD45R (B220), CD45RA, CD45RB, and CD45RO. It is reported that clones 2D1 and HI30 anti-human CD45 clones can reciprocally block the binding of each other to CD45.

PRODUCT DETAILS

Clone: 2D1

Specificity: CD45 

Reactivity: Human

Format: cFluor^® R840^{1, 2}

Isotype: Mouse IgG1, κ

Tested Dilution: 5 µL / test

Application: Flow Cytometry

Storage: 2-8°C and protected from light. Do not freeze

Formulation: Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (BSA Country of Origin USA)

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

REFERENCES

1. Knapp W. et al, 1989. Leucocyte typing IV Oxford University Press. p628-634.

For research use only. Not intended for use in diagnostic procedures.

¹ cFluor^® R840 is equivalent to APC-CF^®790T manufactured and provided by Biotium, Inc. under an Agreement between Biotium and Cytek (LICENSEE). The manufacture, use, sale, offer for sale, or import of the product is covered by one or more of the patents or pending applications owned or licensed by Biotium. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel.

² cFluor^® R840 is a tandem dye made with APC. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.