The mouse monoclonal antibody clone WM15 binds to human CD13, a 150-170 kD type II transmembrane glycoprotein also known as aminopeptidase N, APN, and gp150. The antibody recognizes an extracellular epitope. The CD13 antigen is present on most cells of myeloid origin, including granulocytes, monocytes, mast cells, and GM-progenitor cells. Most AML and CML also express it in myeloid blast crisis, and it is expressed in a smaller fraction of lymphoid leukemias. CD13 is also present on fibroblasts, endothelial cells, epithelial cells from renal proximal tubules and intestinal brush border, bone marrow stromal cells, osteoclasts, and cells lining bile duct canaliculi. CD13 is absent from normal lymphocytes, platelets, and erythrocytes. CD13 is reported to have many different functions, including degradation of enkephalins and endorphins, terminal degradation of peptides, amino acid scavenging, antigen processing, and adhesion and migration of cells. CD13 is also reported to serve as a receptor for some strains of human coronaviruses (HCV).

PRODUCT DETAILS

Clone: WM15

Specificity: CD13 

Reactivity: Human, Baboon, Chimpanzee, Cynomolgus macaque, Rhesus

Format: cFluor^® BYG610^{1, 2}

Isotype: Mouse IgG1, κ

Tested Dilution: 5 µL / test

Application: Flow Cytometry

Storage: 2-8°C and protected from light. Do not freeze

Formulation: Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (BSA Country of Origin USA)

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product

REFERENCES

1. Favaloro EJ, et al. 1987. Dis Markers. 5(4):215-225.
2. Nakamura Y, et al. 1994. Blood. 83(5):1442-3.
3. Freeman SD, et al. 1995. Blood. 85(8):2005-2012.
4. Walter RB, et al. 2007. Traffic. 9(2):267-279.

For research use only. Not intended for use in diagnostic procedures.

¹ cFluor^® BYG610 is equivalent to RPE-CF^®596R manufactured and provided by Biotium, Inc. under an Agreement between Biotium and Cytek (LICENSEE). The manufacture, use, sale, offer for sale, or import of the product is covered by one or more of the patents or pending applications owned or licensed by Biotium. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel.

² cFluor^® BYG610 is a tandem dye made with R-PE. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.