The mouse monoclonal antibody clone ICRF44 binds to human CD11b, also known as alpha M, α-M, ITGAM/CR3A, or Mac-1. This 165-170 kDa cell surface glycoprotein is part of a family of integrin αreceptors that mediate adhesion between cells (cell-cell) and components of the extracellular matrix, e.g., fibrinogen (cell-matrix). In addition, integrin αs are active signaling receptors that recruit leukocytes to inflammatory sites and promote cell activation. Complete, functional integrin αreceptors consist of distinct combinations of integrin αchains, which are differentially expressed. integrin αalpha M (CD11b) assembles with integrin αbeta-2 (CD18) into a receptor known as Macrophage Antigen-1 (Mac-1) or complement receptor type 3 (CR3). This receptor binds and induces intracellular signaling through ICAM-1, ICAM-2, ICAM-3, and ICAM-4 on endothelial cells and can also facilitate the removal of iC3b-bearing foreign cells. CD11b is expressed on many cell types, including myeloid-lineage cells such as monocytes/macrophages, neutrophils, eosinophils, and basophils, and in lymphoid cells such as NK cells, peritoneal B-1 cells, Memory B cells, and microglia.

PRODUCT DETAILS

Clone: ICRF44

Specificity: CD11b 

Reactivity: Human, Baboon, Chimpanzee, Cynomolgus macaque, Rhesus, Swine (Pig)

Format: cFluor^® B532^*

Isotype: Mouse IgG1, κ

Tested Dilution: 5 µL / test

Application: Flow Cytometry

Storage: 2-8°C and protected from light. Do not freeze

Formulation: Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (BSA Country of Origin USA)

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

REFERENCES

1. Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York.
2. Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego.
3. Marsik C, et al. 2003. Shock 20:493-6.; Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97-100.

For research use only. Not intended for use in diagnostic procedures.

^*cFluor^® B532 is equivalent to CF^®503R manufactured and provided by Biotium, Inc. under an Agreement between Biotium and Cytek (LICENSEE). The manufacture, use, sale, offer for sale, or import of the product is covered by one or more of the patents or pending applications owned or licensed by Biotium. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel.