The mouse monoclonal antibody clone 3G3 binds to mouse Foxp3, a 50-55 kDa transcription factor, which is a central regulator of T cell activity and is critical for the development and function of regulatory T cells (Tregs). Foxp3 is expressed at constitutively high levels in Treg cells, which are further identified as being CD4+ CD25+. In resting conventional T cells (CD4+ CD25-) Foxp3 expression is restricted and, upon TCR activation, it is expressed only transiently and in a small proportion of cells. However, the growth factor TGF-beta has been shown to induce the expression of Foxp3 in naive T cells, driving their development into Foxp3+ Tregs, which are called induced or adaptive Tregs. These cells are phenotypically similar to so-called natural Tregs (CD4+ CD25high Foxp3+), which originate in the thymus and comprise the majority of Treg cells. Tregs are critical for maintaining peripheral tolerance and are implicated in the development of autoimmunity. Forced expression of Foxp3 in conventional T cells results in the upregulation of Treg-associated molecules such as CD25, CTLA-4, and GITR, and is sufficient to impart suppressive functional activity to these cells. It is important to review the literature in choosing an antibody for the Foxp3 antigen in flow cytometry, as the potential for high background or non-specific staining may be observed.

PRODUCT DETAILS

Clone: 3G3

Specificity: Foxp3

Reactivity: Mouse

Format: Purified

Isotype: Mouse IgG1, k

Application: WB, FC-IC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Western blotting: Recommended dilution: 2 μg/ml.
Flow cytometry: Recommended dilution: 1-4 µg/ml. Intracellular staining.

REFERENCES

1. Cole DK, et al. 2004. Cell Mol Immunol. 1(2):81-8.
2. Kern Petra S, et al. 1998. Immunity. 9: 519-530.
3. Veillette A. et al. 1988. Cell. 55:301-308.

For research use only. Not intended for use in diagnostic procedures.