The rat monoclonal antibody clone M17/4 binds to mouse CD11a, a 180kD glycoprotein that is also known as integrin alpha L (ITGAL). CD11a associates non-covalently with CD18 (integrin beta 2) to form the lymphocyte function associated with antigen-1 (LFA-1) heterodimer. CD11a is expressed on the cell surface of all leukocytes. LFA-1 plays a critical role in intercellular adhesion that is mediated through interactions with its ligands, ICAM-1 (CD54), ICAM-2 (CD102), and ICAM-3 (CD50). LFA-1 also functions in lymphocyte costimulatory signaling. On resting cells, LFA-1 exists in a low-affinity conformation that is rapidly induced to the high-affinity conformation following cell stimulation.

PRODUCT DETAILS

Clone: M17/4

Specificity: CD11a

Reactivity: Mouse

Format: Purified

Isotype: Rat IgG2a, k

Application: FC, IP

Storage: 2-8°C protected from light.Do not freeze

Formulation: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Flow cytometry: Recommended dilution: 1 μg/ml.
Immunohistochemistry (frozen sections): Positive tissue: murine spleen or thymus, acetone fixation.

For research use only. Not intended for use in diagnostic procedures.