The antibody NF-01 recognizes a phosphorylated epitope on heavy neurofilament protein (210 kDa intracellular antigen) of various species. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons and are biochemically very stable.

PRODUCT DETAILS

Clone: NF-01

Specificity: NF-H

Reactivity: Mammal

Format: Purified

Isotype: Mouse IgG1

Application: IHC-P, WB, ICC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Immunohistochemistry (paraffin sections): Recommended dilution: 5-10 μg/ml.
Western blotting: Recommended dilution: 1-2 μg/ml.

REFERENCES

1. Côté F, et al. 1993. Cell 73:35-46.
2. Ohara O, et al. 1993. J Cell Biol 121:387-95.
3. Wild EJ, et al. 2007. Neurosci Lett 417:231-3.
4. Miyazawa I, et al. 2007. Neurology 68:865-7.
5. Petzold A, et al. 2007. Neurodegener Dis 4:185-94.
6. Lukás Z, et al. 1993. Histochemistry 100:495-502.

For research use only. Not intended for use in diagnostic procedures.