The antibody VI-10 reacts with vimentin, a 57 kDa intermediate filament intracellular protein expressed in variety of mesenchymal and mesodermal cell types.

PRODUCT DETAILS

Clone: VI-10

Specificity: Vimentin

Reactivity: Human|Mouse, Chicken, Rat, Pig

Format: Purified

Isotype: Mouse IgM

Application: IP, WB, IHC-P, ICC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Immunocytochemistry: Staining technique: (a) fix cells for 10 min in methanol at -20°C and for 6 min in acetone at -20°C; (b) fix cells directly in methanol for 10 min at -20°C or in acetone for 10 min at -20°C. Positive control: 3T3 murine Swiss albino fibroblast cell line, RBL rat basophilic leukemia cell line.
Immunohistochemistry (paraffin sections): Recommended dilution: 5 μg/ml, positive tissue: skin fibroblast.
Western blotting: Recommended dilution: 1-2 μg/ml.

REFERENCES

1. Lukás Z, et al. 1989. Histochem J 21:693-701.
2. Lukás Z, et al. 1993. Histochemistry 100:495-502.

For research use only. Not intended for use in diagnostic procedures.