The mouse monoclonal antibody GCP-04 recognizes amino acids 100-104 of extracellular domain of denaturated glutamate carboxypeptidase II (PSMA, NAALADase, FOLH1), an approximately 95-110 kDa transmembrane glycoprotein.

PRODUCT DETAILS

Clone: GCP-04

Specificity: PSMA

Reactivity: Human|Mouse, Pig, Rat

Format: Purified

Isotype: Mouse IgG1

Application: WB, IHC-P, ICC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Western blotting: Recommended dilution: 1 μg/ml; positive control: LNCaP cell line. Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold lysis buffer (1% NP-40). Incubate 30 min on ice. Mix lysate with non-reducing/reducing Laemmli SDS-PAGE sample buffer. Both reducing and non-reducing conditions.

REFERENCES

1. Ghadge GD, et al. 2003. Proc Natl Acad Sci U S A 100:9554-9.
2. Mesters JR, et al. 2006. EMBO J 25:1375-84.
3. Davis MI, et al. 2005. Proc Natl Acad Sci U S A 102:5981-6.
4. Barinka C, et al. 2004. Eur J Biochem 271:2782-90.
5. Barinka C, et al. 2004. Protein Sci 13:1627-35.
6. Sácha P, et al. 2007. Neuroscience 144:1361-72.
7. Rovenská M, et al. 2008. Prostate 68:171-82.
8. Barinka C, et al. 2002. J Neurochem 80:477-87.
9. Tykvart J, et al. 2014. Prostate 74:1674-90.

For research use only. Not intended for use in diagnostic procedures.