The mouse monoclonal antibody FYN-01 recognizes Fyn, a 59 kDa non-receptor Src-family protein tyrosine kinase (intracellular antigen).

PRODUCT DETAILS

Clone: FYN-01

Specificity: FYN

Reactivity: Human|Mouse

Format: Purified

Isotype: Mouse IgG2b

Application: IP, WB, IHC-P, ICC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Immunohistochemistry (paraffin sections): Recommended dilution: 5 μg/ml; positive tissue: brain.
Western blotting: Recommended dilution: 2 μg/ml; positive control: JURKAT human T cell leukemia cell line.
Immunoprecipitation: positive control: J77 T cell line. Preparation of cell lysate: 30 min on ice (orbital incubator) in lysing buffer, lysing buffer with N-dodecyl beta-D-maltoside (20 mM Tris/Cl, 100 mM NaCl pH 8,2, 1% laurylmaltosid (w/v), 50 mM NaF). The antibody FYN-01 apparently gives very specific signal (by 59 kDa), and nicely immunoprecipitates Fyn from cell lysates.

REFERENCES

1. Rivera J, et al. 2008. Curr Allergy Asthma Rep 8:14-20.
2. Makinodan E, et al. 2007. Neuroscience 147:1-4.
3. Samayawardhena LA, et al. 2007. Blood 109:3679-86.
4. Filby A, et al. 2007. J Immunol 179:4635-44.
5. Wu HY, et al. 2007. J Biol Chem 282:20075-87.
6. Hattori K, et al. 2006. J Biol Chem 281:7129-35.
7. Talmor-Cohen A, et al. 2004. Reproduction 128:387-93.
8. Utting O, et al. 2001. J Immunol 166:1540-6.
9. Meraner P, et al. 2007. J Immunol 178:2262-71.

For research use only. Not intended for use in diagnostic procedures.