The antibody MEM-E/06 recognizes an extracellular epitope on native surface-expressed HLA-E, but not denaturated heavy chain of HLA-E. HLA-E belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on many types of the human cells. The published results showed that the antibody cross-reacts with some classical MHC Class I molecules (HLA-A3, -A11, -B7). However, the recent Workshop I Session on the 3rd International Conference on HLA-G (Paris, July 2003) confirmed that the antibody exhibits much broader cross-reactivity classical MHC Class I antigens, namely with HLA-A24, -A32, -B8, -B15, -B27, -B35, -B44, -B54, -C3, -C4, -C5, -C7.

PRODUCT DETAILS

Clone: MEM-E/06

Specificity: MHC Class I (HLA-E)

Reactivity: Human, Non-Human Primate

Format: Purified

Isotype: Mouse IgG1

Application: FC, IP, IHC-P

Storage: 2-8°C protected from light.Do not freeze

Formulation: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: spleen.
Flow cytometry: Recommended dilution: 1-4 µg/ml

REFERENCES

1. Dambaeva SV, et al. 2008. Placenta 29:58-70.
2. Gonen-Gross T, et al. 2005. J Immunol 175:4866-74.
3. Palmisano GL, et al. 2005. Hum Immunol 66:1-12.
4. Menier C, et al. 2003. Hum Immunol 64:315-26.

For research use only. Not intended for use in diagnostic procedures.