The mouse monoclonal antibody DE-K13 reacts with cytokeratin 10 (56.5 kDa) and cytokeratin 13 (53 kDa). Cytokeratins are members of intermediate filaments subfamily of intracellular proteins, represented in epithelial tissues. DE-K13 recognizes only cytokeratin 13 on formalin-fixed, paraffin-embedded tissue sections.

PRODUCT DETAILS

Clone: DE-K13

Specificity: Cytokeratin 10, 13

Reactivity: Human, Feline

Format: Purified

Isotype: Mouse IgG2a

Application: WB, IHC-P

Storage: 2-8°C protected from light.Do not freeze

Formulation: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Immunohistochemistry (paraffin sections): Standard ABC technique (DAB+), recommended dilution: 10 μg/ml (1:100), positive tissue: tonsil. Pretreatment: 0.1% pepsin in 0.1 M HCl for 30 min at room temperature or Target Retrieval Solution - High pH. The antibody DE-K13 reacts exclusively with Cytokeratin 13.
Immunohistochemistry (frozen sections): Use at an assay dependent dilution.
Western blotting: recommended dilution: 1-3 μg/ml.

REFERENCES

1. Ivanyi D, et al. 1993. Am J Vet Res 54:1095-102.
2. Ivanyi D, et al. 1993. Am J Vet Res 54:1095-102.
3. Kozaki M, et al. 2001. J Vet Med Sci 63:1-4.

For research use only. Not intended for use in diagnostic procedures.