Purified Anti-Human CD66e (CB30)
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Description
The mouse monoclonal antibody CB30 recognizes CD66e (CEA; 180-200 kDa), an extracellular cell surface-bound carcinoembryonic antigen mainly expressed on epithelial cells.
PRODUCT DETAILS
Clone: CB30
Specificity: CD66e
Reactivity: Human
Format: Purified
Isotype: Mouse IgG1
Application: FC, IP, IHC-P
Storage: 2-8°C protected from light.Do not freeze
Formulation: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
RECOMMENDED USAGE
Immunohistochemistry (paraffin sections): Staining technique: standard ABC technique (DAB+), recommended dilution: 10 μg/ml (1:100), positive tissue: adenocarcinoma of colon, pretreatment: 0,1% pepsin in 0,1 M HCl for 30 min at room temperature.
REFERENCES
- Oikawa S, et al. 1987. Biochem Biophys Res Commun 142:511-8.
- Thompson JA, et al. 1991. J Clin Lab Anal 5:344-66.
- Gangopadhyay A, et al. 1996. Cancer Res 56:4805-10.
- Chen CJ, et al. 1995. Cancer Res 55:3873-82.
- Koga S, et al. 2014. Cancer Sci 105:1299-306.
- Wang L, et al. 2016. Oncoimmunology 5:e1211218.
For research use only. Not intended for use in diagnostic procedures.