The antibody MEM-111 reacts with an extracellular epitope of CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein (receptor for rhinovirus). The expression of CD54 is upregulated by activation; it is expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells.

PRODUCT DETAILS

Clone: MEM-111

Specificity: CD54 (ICAM1)

Reactivity: Human, Bovine, Rat

Format: Purified

Isotype: Mouse IgG2a

Application: FC, WB, IHC-P, ELISA, ICC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Flow cytometry: Recommended dilution: 0.5-4 μg/ml.
Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: thymus, RE cells.
Western blotting: Recommended dilution: 1-2 μg/ml; non-reducing conditions.

REFERENCES

1. Boyd AW, et al. 1988. Proc Natl Acad Sci U S A 85:3095-9.
2. Boyd AW, et al. 1989. Blood 73:1896-903.
3. Springer TA. 1990. Nature 346:425-34.
4. Ockenhouse CF, et al. 1992. Cell 68:63-9.
5. Nagaraju K, et al. 1998. Clin Exp Immunol 113:407-14.
6. Tachimoto H, et al. 2002. Am J Respir Cell Mol Biol 26:645-9.
7. Bacáková L, et al. 2000. Biomaterials 21:1173-9.
8. Lee DJ, et al. 2007. J Immunol 179:8128-36.
9. Bacáková L, et al. 2000. J Biomed Mater Res 49:369-79.
10. Leeuwenberg JF, et al. 1992. Immunology 77:543-9.
11. Lee DJ, et al. 2007. J Immunol 179:8128-36.

For research use only. Not intended for use in diagnostic procedures.