The antibody MEM-04 reacts with an extracellular epitope of CD50 (ICAM-3), a 120-130 kDa type I membrane protein (immunoglobulin supergene family) expressed on leukocytes, endothelial cells and Langerhans cells; it is negative on platelets and erythrocytes. The MEM-04 blocks DC-SIGN (CD209) binding to ICAM-3 (epitope in D1).

PRODUCT DETAILS

Clone: MEM-04

Specificity: CD50

Reactivity: Human

Format: Purified

Isotype: Mouse IgG2b, k

Application: FC, IHC-P, FUNC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Functional application: The antibody MEM-04 blocks DC-SIGN (CD209) binding to ICAM-3 (epitope in D1).
Flow cytometry: Recommended dilution: 1-4 μg/ml.
Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: tonsil.

REFERENCES

1. Campanero MR, et al. 1993. J Cell Biol 123:1007-16.
2. Hernandez-Caselles T, et al. 1993. Eur J Immunol 23:2799-806.
3. Arroyo AG, et al. 1994. J Cell Biol 126:1277-86.
4. Bogoevska V, et al. 2007. Glycobiology 17:324-33.

For research use only. Not intended for use in diagnostic procedures.