The antibody MEM-143 reacts with an extracellular epitope of CD45RB, a 180-240 kDa single chain type I membrane glycoprotein, variant of CD45 (CD45RB isoform). CD45RB is expressed on a subset of T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells. The MEM-143 is therefore not neuraminidase sensitive like most other anti-CD45RB antibodies (including standard antibody MEM-55). The reactivity of the antibody MEM-143 can be blocked by a peptide including amino acids 79-88.

PRODUCT DETAILS

Clone: MEM-143

Specificity: CD45RB

Reactivity: Human

Format: Purified

Isotype: Mouse IgG1

Application: FC, IHC-P, WB

Storage: 2-8°C protected from light.Do not freeze

Formulation: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Flow cytometry: Recommended dilution: 0.5-4 μg/ml. The staining pattern of the MEM-143 shows prominent differences from other anti-CD45RB reagents including standard antibody MEM-55, mostly as a result of the recognition of differences in glycosylation.
Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive control: human tonsil.
Western blotting: Recommended dilution: 1-2 μg/ml.

REFERENCES

1. Townsend KP, et al. 2004. Neurosci Lett 362:26-30.
2. Li FJ, et al. 2005. Blood 105:3295-302.
3. Cosenza-Nashat MA, et al. 2006. Brain Pathol 16:256-65.
4. Dawes R, et al. 2006. J Immunol 176:3417-25.
5. Bijian K, et al. 2007. Mol Immunol 44:3682-90.
6. Desharnais P, et al. 2008. Apoptosis 13:197-212.

For research use only. Not intended for use in diagnostic procedures.