The mouse monoclonal antibody clone HIP1 reacts with human CD42b, also known as GPIbα (glycoprotein Ibα), a 135–145 kDa type I transmembrane glycoprotein and member of the leucine-rich repeat (LRR) family. CD42b forms a disulfide-linked heterodimer with CD42c (GPIbβ), which associates noncovalently with CD42a (GPIX) and CD42d (GPV) to constitute the GPIb-IX-V receptor complex on the platelet surface. The extracellular domain of CD42b contains eight leucine-rich repeats and an anionic region with sulfated tyrosines that collectively mediate binding to von Willebrand factor (VWF) exposed at sites of vascular injury, initiating platelet tethering and rolling under high shear conditions. CD42b also binds thrombin, coagulation factors XI and XII, high molecular weight kininogen, P-selectin, and integrin Mac-1 (CD11b/CD18). CD42b expression is restricted to platelets and megakaryocytes in bone marrow. Mutations and polymorphisms in CD42b are associated with Bernard-Soulier syndrome, a congenital bleeding disorder, and platelet-type von Willebrand disease. The HIP1 clone recognizes an epitope within the N-terminal region of the GPIbα chain and has been reported to inhibit ristocetin-dependent VWF binding and ristocetin-induced platelet agglutination. This antibody is reported for use in flow cytometric analysis, western blotting, immunohistochemical staining of frozen tissue sections, and in vitro functional blocking assays.

PRODUCT DETAILS

Clone: HIP1

Specificity: CD42b

Reactivity: Human

Format: Purified

Isotype: Mouse IgG1, k

Application: FC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Flow cytometry: Recommended dilution: 1-4 µg/ml

REFERENCES

1. Ruggeri, Z.M. 1997. J. Clin. Invest. 99: 559–564.
2. Lenting, P.J. et al. 2015. Cell. Mol. Life Sci. 72: 187–204.
3. Rauch, A. et al. 2022. J. Thromb. Haemost. 20: 1GP1–1GP15.

For research use only. Not intended for use in diagnostic procedures.