The antibody MEM-115 recognizes an extracellular epitope in the D1 domain of CD4 antigen, a 55 kDa transmebrane glycoprotein expressed on a subset of T lymphocytes (“helper“ T cells) and also on monocytes, tissue macrophages and granulocytes. It is negative in Western blotting even with non-reduced samples of cell lysates.

PRODUCT DETAILS

Clone: MEM-115

Specificity: CD4

Reactivity: Human

Format: Purified

Isotype: Mouse IgG2a

Application: IP, FC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Immunoprecipitation: Excellent.
Flow cytometry: Recommended dilution: 3 μg/ml. Although it has not been tested rigorously, following data suggest that the antibody MEM-115 is a low-affinity antibody: its binding to T cells increases at elevated temperature, monovalent Fab fragments essentially do not bind to T cells.

REFERENCES

1. Millán J, et al. 1999. Tissue Antigens 53:33-40.
2. Bosze S, et al. 2004. Biopolymers 76:467-76.
3. Singer II, et al. 2001. J Virol 75:3779-90.
4. Brdicková N, et al. 2003. J Exp Med 198:1453-62.

For research use only. Not intended for use in diagnostic procedures.