The mouse monoclonal antibody clone 4H11 binds to human CD34, also known as mucosialin. CD34 belongs to a protein family, which also includes endoglycan and podocalyxin. Members of this family are single-pass transmembrane proteins with a heavily glycosylated extracellular and N-terminal mucin domain. CD34 was first identified as an antigen expressed on hematopoietic progenitors and has since been extensively used as a marker to isolate cells capable of hematopoietic cell engraftment. Despite this, the function of CD34 remains unresolved. In addition to expression on hematopoietic progenitors, CD34 is expressed on some populations of mesenchymal stem cells, tumor cell lines, and by vascular endothelia in the adult. Epitopes of CD34 have been assigned to three classes (class I, II or III) based on their differential sensitivity to enzymatic cleavage by neuraminidase, chymopapain, or O-glycoprotease. The 4H11 clone belongs to class III, indicating that it reacts with a protein epitope on CD34.

PRODUCT DETAILS

Clone: 4H11

Specificity: CD34

Reactivity: Human

Format: Purified

Isotype: Mouse IgG1, k

Application: FC, WB, IHC-P, ICC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Western blotting: Recommended dilution: 1-2 μg/ml; positive control: Kg-1a, TF-1 cells, non-reducing conditions.
Flow cytometry: Recommended dilution: 2 μg/ml.
Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: placenta endothelium.

For research use only. Not intended for use in diagnostic procedures.