The mouse monoclonal antibody Vu1D9 recognizes an extracellular epitope within EGF-like domain I of CD326 / EpCAM, a marker of epithelial lineages. This antibody strongly stains various normal epithelial cells and carcinomas.

PRODUCT DETAILS

Clone: Vu1D9

Specificity: CD326 (Ep-CAM)

Reactivity: Human

Format: Purified

Isotype: Mouse IgG1

Application: FC, IHC-P, IP, WB, ICC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Immunohistochemistry (paraffin sections): Recommended dilution: 5-10 μg/ml; positive control: colon epithelium, antigen retrieval: heat (sodium citrate) + trypsin.
Immunocytochemistry: Recommended dilution: 4 μg/ml.
Western blotting: Recommended dilution: 1-4 μg/ml, non-reducing conditions.
Flow cytometry: Recommended dilution: 1-3 μg/ml.
Immunoprecipitation: Recommended dilution: 1-2 μg / 100-500 μg of protein in 1 ml lysate.

REFERENCES

1. Brunner A, et al. 2008. J Clin Pathol 61:307-10.
2. Winter MJ, et al. 2003. Am J Pathol 163:2139-48.
3. Li G, et al. 2000. Anticancer Res 20:2773-8.
4. Ogura E, et al. 1998. Anticancer Res 18:3669-75.
5. Tsubura A, et al. 1992. J Cutan Pathol 19:73-9.

For research use only. Not intended for use in diagnostic procedures.