The mouse monoclonal antibody 323/A3 recognizes an extracellular epitope of CD326 / EpCAM, a marker of epithelial lineages, that is over-expressed in many carcinomas.

PRODUCT DETAILS

Clone: 323/A3

Specificity: CD326 (Ep-CAM)

Reactivity: Human

Format: Purified

Isotype: Mouse IgG1

Application: FC, IP, WB, IHC-P, ICC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Western blotting: Non-reducing conditions; recommended dilution: 1-2 μg/ml; positive control: MCF-7 cells.
Immunoprecipitation: Recommended dilution: 1-4 μg / ml per 100-500 μg of protein in 1 ml lysate.
Immunohistochemistry (paraffin sections): Recommended dilution: 1-10 μg/ml; pretreatment: tissue section digestion with pepsin (15 min at RT or 10 min at 37°C, 1 mg / ml Tris-HCl, pH 2.0); positive tissue: breast carcinoma.
Immunocytochemistry: Recommended dilution: 1-10 μg/ml.
Flow cytometry: Recommended dilution: 1-4 µg/ml.

REFERENCES

1. Kirman I, et al. 2003. Dig Dis Sci 48:2306-9.
2. Winter MJ, et al. 2003. Am J Pathol 163:2139-48.
3. Balzar M, et al. 2001. Mol Cell Biol 21:2570-80.
4. Balzar M, et al. 1998. Mol Cell Biol 18:4833-43.
5. Litvinov SV, et al. 1994. Cell Adhes Commun 2:417-28.
6. Litvinov SV, et al. 1994. J Cell Biol 125:437-46.
7. Edwards DP, et al. 1986. Cancer Res 46:1306-17.

For research use only. Not intended for use in diagnostic procedures.