The antibody MEM-174 recognizes an extracellular epitope of CD11b antigen (Mac-1 alpha), a 165-170 kDa type I transmembrane protein mainly expressed on monocytes, granulocytes and NK-cells. The CD11b mediates neutrophil and monocyte interactions with stimulated endothelium.

PRODUCT DETAILS

Clone: MEM-174

Specificity: CD11b

Reactivity: Human

Format: Purified

Isotype: Mouse IgG2a

Application: FC, IP

Storage: 2-8°C protected from light.Do not freeze

Formulation: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Flow cytometry: Recommended dilution: 1 μg/ml; positive control: peripheral blood lymphocytes.
Immunoprecipitation: Positive control: granulocytes, CD11b/CD18 transfectants.
Immunoprecipitation with the antibody MEM-174 was successfuly performed using immuno-affinity sorbents (minicolumns). The final immunosorbent should contain 1-5 mg of antibody/1 ml of sorbent gel (e.g. agarose beads). It is recommended to stimulate positive control material by LPS 1-2 days before to achieve better expression of target antigen.

REFERENCES

1. Hentzen ER, et al. 2000. Blood 95:911-20.
2. Lawrence PK, et al. 2007. Vet Immunol Immunopathol 119:287-98.
3. Akramiene D, et al. 2007. Medicina (Kaunas) 43:597-606.
4. Drbal K, et al. 2007. Int Immunol 19:675-84.
5. Hasan S, et al. 2015. FEBS Lett 589:374-9.

For research use only. Not intended for use in diagnostic procedures.