The mouse monoclonal antibody B2M-02 reacts with beta2-microglobulin (beta2M), an extracellular antigen associated with cell-surface MHC Class I molecules and other membrane antigens, as well as it reacts with soluble beta2-microglobulin. Beta2M is a 12 kDa Ig like glycoprotein expressed on lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells and epithelial cells. It is absent on erythrocytes.

PRODUCT DETAILS

Clone: B2M-02

Specificity: Beta-2 Microglobulin

Reactivity: Human, Pig

Format: Purified

Isotype: Mouse IgG1

Application: FC, WB, IHC-P, ELISA, ICC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Immunohistochemistry (paraffin sections): Heat-mediated antigen retrieval; recommended dilution: 5 μg/ml; positive tissue: liver.
Western blotting: Recommended dilution: 2 μg/ml; non-reducing conditions preferred.
Flow cytometry: Recommended dilution: 1 μg/ml; positive control: PBL cell line, negative control: DAUDI human lymphoma cell line, erythrocytes.
ELISA: Working dilution should be determinated by investigator.

REFERENCES

1. Drbal K, et al. 2001. Blood 98:1561-6.
2. Hilgert I, et al. 1984. Folia Biol (Praha) 30:369-76.

For research use only. Not intended for use in diagnostic procedures.