The mouse monoclonal antibody clone L243 binds to the human HLA-DR antigen, a 36 kDa alpha heavy chain, and a 27 kDa beta light chain heterodimeric cell surface glycoprotein in the transmembrane major histocompatibility complex 2 (MHC II) family. HLA-DR, also known as human leukocyte antigen DR isotype, is present on the surface of antigen-presenting cells including, B cells, dendritic cells, macrophages, monocytes, and activated T cells. MHC class II regulates the immune system by playing a critical role in binding and presenting antigen-derived peptides to peptide-MHC II-specific CD4 T cells. The L243 clone identifies HLA-DR molecules, whether peptide-loaded or unoccupied. It does not exhibit cross-reactivity with HLA-DP and HLA-DQ. Clone L243 interacts with a conformational epitope on HLA-DRα, contingent upon the proper folding of the αβ heterodimer.

PRODUCT DETAILS

Clone: L243

Specificity: HLA-DR

Reactivity: Human, African Green Monkey, Baboon, Chimpanzee, Cynomolgus Monkey, Dog, Common Marmoset, Rhesus Macaque, Squirrel Monkey

Format: PE-Cyanine5

Isotype: Mouse IgG2a, k

Tested Dilution: 5 µL/Test

Application: FC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Flow cytometry: The suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application. The content of a vial (0.5 mL) is sufficient for 100 tests.

REFERENCES

1. Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York.
2. Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego.
3. Marsik C, et al. 2003. Shock 20:493-6.
4. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97-100.

For research use only. Not intended for use in diagnostic procedures.

PE-Cyanine5 is a tandem dye made with PE. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.