The mouse monoclonal antibody clone 2H7 binds to human CD20. CD20 is a phosphoprotein with a molecular weight of 33-37 kDa and has four transmembrane domains. It is present at all stages of B cell development, except for early pro-B cells and plasma cells, and plays a crucial role in B cell activation, proliferation, and differentiation. In some studies, CD20 is thought to act as a Ca2+ channel in the plasma membrane. It is also present on a T cell subset at low levels and some follicular dendritic cells.

PRODUCT DETAILS

Clone: 2H7

Specificity: CD20

Reactivity: Human, Baboon, Capuchin Monkey, Chimpanzee, Cynomolgus Monkey, Pigtail Monkey, Rhesus Macaque, Squirrel Monkey

Format: PE-Cyanine5

Isotype: Mouse IgG2b, k

Tested Dilution: 5 µL/Test

Application: FC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Flow cytometry: The suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application. The content of a vial (0.5 mL) is sufficient for 100 tests.

REFERENCES

1. Ledbetter et al. 1986. Hum Immunol. 15: 30-43.

For research use only. Not intended for use in diagnostic procedures.

PE-Cyanine5 is a tandem dye made with PE. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.