The mouse monoclonal antibody clone 15C4 binds to the human lectin-like oxidized LDL receptor 1 (LOX-1), also known as CLEC8A. LOX-1 is a 31 kDa single-pass type II transmembrane protein and a member of the C-type lectin superfamily. It is a scavenger receptor that is involved in endocytosis, phagocytosis, and cytokine production. LOX-1 is expressed by endothelial cells, smooth muscle cells, platelets, fibroblasts, and macrophages, and is upregulated by inflammatory and oxidative stimuli. LOX-1 recognizes numerous ligands, including lipoproteins, apoptotic cells, heat shock proteins, C-reactive protein, and oxidized LDL.

PRODUCT DETAILS

Clone: 15C4

Specificity: LOX-1

Reactivity: Human

Format: PE

Isotype: Mouse IgG2a, k

Tested Dilution: 5 µL/Test

Application: FC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Flow cytometry: The suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application. The content of a vial (0.5 mL) is sufficient for 100 tests.

REFERENCES

1. Li D, et al. 2012. J Exp Med. 209:109-121.
2. Joo, H., et. al. 2014. Immunity. 41:592-604.

For research use only. Not intended for use in diagnostic procedures.