The antibody MEM-171 recognizes an extracellular epitope in the D2 domain of CD50 (ICAM-3), a 120-130 kDa type I membrane protein (immunoglobulin supergene family) expressed on leukocytes, endothelial cells and Langerhans cells; it is negative on platelets and erythrocytes.

PRODUCT DETAILS

Clone: MEM-171

Specificity: CD50

Reactivity: Human

Format: PE

Isotype: Mouse IgG1

Tested Dilution: 5 µL/Test

Application: FC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Flow cytometry: The suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application. The content of a vial (0.5 mL) is sufficient for 100 tests.

REFERENCES

1. Campanero MR, et al. 1993. J Cell Biol 123:1007-16.
2. Hernandez-Caselles T, et al. 1993. Eur J Immunol 23:2799-806.
3. Arroyo AG, et al. 1994. J Cell Biol 126:1277-86.
4. Bogoevska V, et al. 2007. Glycobiology 17:324-33.
5. Linnebacher M, et al. 2010. J Biomed Biotechnol 2010:841451.
6. Filatov AV, et al. 2007. J Immunol Methods 319:21-33.

For research use only. Not intended for use in diagnostic procedures.