The Armenian hamster monoclonal antibody clone N418 binds to mouse CD11c, also known as integrin alpha X. This 150 kDa cell surface glycoprotein is part of a family of integrin receptors that mediate adhesion between cells (cell-cell) and components of the extracellular matrix, e.g. fibrinogen (cell-matrix). In addition, integrins are active signaling receptors that recruit leukocytes to inflammatory sites and promote cell activation. Complete, functional integrin receptors consist of distinct combinations of integrin chains that are differentially expressed. Integrin alpha X (CD11c) assembles with Integrin beta-2 (CD18) into a receptor complex known as CR4, which can bind and induce signaling through ICAMs and VCAM-1 on endothelial cells and can also facilitate the removal of iC3b-bearing foreign cells. CD11c is prominently expressed on tissue macrophages and detected on some types of activated T cells and intestinal intraepithelial lymphocytes (IEL).

PRODUCT DETAILS

Clone: N418

Specificity: CD11c

Reactivity: Mouse

Format: cFluor^® V435

Isotype: Armenian Hamster IgG

Tested Dilution: 5 µl/Test

Application: Flow Cytometry

Storage: 2-8°C protected from light. Do not freeze

Formulation: Phosphate-buffered saline, pH 7.2, containing 0.1% PEG 3350, 0.1% Pluronic F-68, 0.05% Tween-20, 0.09% sodium azide and 1% BSA (BSA Country of Origin USA)

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 μL per 1 million cells in a staining volume of 100 μL. If whole blood is analyzed, then use 5 μL per 100 μL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

For research use only. Not intended for use in diagnostic procedures.

Cytek^® cFluor^® V435 is StarBright^™ Violet 440 Dye, manufactured and provided by Bio-Rad, Inc.

StarBright is a trademark of Bio-Rad Laboratories, Inc. in certain jurisdictions. StarBright Dyes are manufactured and provided by Bio-Rad under an Agreement between Bio-Rad supplied to Cytek, being subject to certain conditions.