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cFluor<sup>®</sup> R668 Anti-Human CD314 (NKG2D) (1D11)

cFluor® R668 Anti-Human CD314 (NKG2D) (1D11)

SKU R7-20472
Regular price

$137.20

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Description

The 1D11 monoclonal antibody binds to human CD314, a homodimeric C-type lectin-like protein also known as NKG2D. It is expressed on NK cells, CD8 T cells, γ/δ T cells, and in vitro-induced LAK cells. Several molecules have been identified as the ligands for NKG2D, including MHC class-I chain-related protein A (MICA), MICB, and UL16-binding proteins (ULBPs). NKG2D has no intrinsic signaling capacity, but participate in signaling this by non-covalent association with DAP10 or DAP12 adaptors. In addition to being a primary activation receptor on NK cells, NKG2D is also a costimulatory receptor for TCR-mediated T cell proliferation and cytokine production. The interaction of NKG2D with its ligands plays a role in immune surveillance against pathogen and tumor cells, as well as in the pathogenesis of autoimmune diseases.

The antibody was conjugated to a fluorophore and purified by affinity chromatography.


PRODUCT DETAILS

Clone:          1D11

Reactivity:     Human, African Green, Baboon, Cynomolgus, Rhesus

Format:          cFluor® R668*

Isotype:              Mouse IgG1, κ

Tested Dilution:  5 μL / test

Application:        Flow Cytometry

Storage:               2-8°C and protected from Light. Do not freeze

Formulation:       Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (Origin USA)

Data Sheets: TDS | SDS

 

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

 

REFERENCES

  1. Wu J, et al. 1999. Science 285:730-732.
  2. Wu J, et al. 2000. J. Exp. Med. 192:1059-1068.
  3. Groh V, et al. 2001. Nature Immunol. 2:255-260.
  4. Wu J, et al. 2002. J. Immunol. 169:1236-1240.
  5. Roberts A, et al. 2001. J. Immunol. 167:5527-5530.
  6. Groh V, et al. 2003. Proc. Natl. Acad. Sci. USA 100:9452-9457.
  7. Kraetzel K et al. 2008. Eur. Respir. J. 32:563-570.
  8. Correia DV, et al. 2011. Blood 118:992-1001.
  9. Watanbe M, et al. 2014. Int Immunol. 26:597-606.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.


*cFluor® R668 is equivalent to CF®647 manufactured and provided by Biotium, Inc. under an Agreement between Biotium and Cytek (LICENSEE). The manufacture, use, sale, offer for sale, or import of the product is covered by one or more of the patents or pending applications owned or licensed by Biotium. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel.


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