The mouse monoclonal antibody clone TS2/9 recognizes human CD58, also known as lymphocyte function-associated antigen-3 (LFA-3). CD58 is a widely expressed 40–70 kDa cell surface glycoprotein belonging to the immunoglobulin superfamily and is present on hematopoietic and non-hematopoietic cells, including lymphocytes, monocytes, dendritic cells, endothelial cells, and epithelial cells. CD58 functions as a key adhesion and costimulatory molecule by binding CD2 on T cells and natural killer (NK) cells, facilitating immune synapse formation, T-cell activation, and cytotoxic effector function. The CD2–CD58 interaction is important in both antigen-dependent and antigen-independent immune responses and contributes to intercellular adhesion and immune regulation. Clone TS2/9 recognizes an extracellular epitope of human CD58 and is suitable for flow cytometric analysis of CD58 expression across hematopoietic cell populations and in studies of immune activation, adhesion biology, and tumor immunology.

PRODUCT DETAILS

Clone: TS2/9

Specificity: CD58

Reactivity: Human

Format: cFluor^® R659

Isotype: Mouse IgG1, k

Tested Dilution: 5 µL/Test

Application: Flow Cytometry

Storage: 2-8°C protected from light. Do not freeze

Formulation: Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (BSA Country of Origin USA)

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 μL per 1 million cells in a staining volume of 100 μL. If whole blood is analyzed, then use 5 μL per 100 μL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

REFERENCES

1. Sanchez-Madrid F, Krensky AM, et al. Proc Natl Acad Sci USA. 1982;79(23):7489-7493.
2. Dustin ML, Sanders ME, et al. J Immunol. 1987;139(1):9-15.
3. Shaw S, Luce GE, et al. Nature. 1986;323(6085):262-264.

For research use only. Not intended for use in diagnostic procedures.