cFluor® BYG750 Anti-Mouse CD8a (53-6.7)
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Description
The rat monoclonal antibody clone 53-6.7 binds to the 32-34 kDa alpha subunit of mouse CD8, known as CD8a or CD8 alpha. CD8a can form a homodimer (CD8 alpha-alpha) but is more commonly expressed as a heterodimer with a second chain known as CD8b or CD8 beta. CD8 acts as a co-receptor in antigen recognition and subsequent T cell activation that is initiated upon binding of the T cell receptor (TCR) to antigen-bearing MHC Class I molecules. The cytoplasmic domains of CD8 provide binding sites for the tyrosine kinase Lck, facilitating intracellular signaling events that lead to T cell activation, development, and cytotoxic effector functions. CD8+ cytotoxic T cells (CTLs) play an important role in inducing cell death of tumor cells, as well as cells infected by viruses, bacteria, or parasites. It can be used as a phenotypic marker for mouse CD8a expression on cytotoxic T cells and thymocytes and on specific cell types that do not also express the TCR, including some NK cells and lymphoid dendritic cells.
PRODUCT DETAILS
Clone: 53-6.7
Specificity: CD8a
Reactivity: Mouse
Format: cFluor® BYG750
Isotype: Rat IgG2a, k
Tested Dilution: 5 µl/Test
Application: Flow Cytometry
Storage: 2-8°C protected from light. Do not freeze
Formulation: Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (BSA Country of Origin USA)
RECOMMENDED USAGE
Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 μL per 1 million cells in a staining volume of 100 μL. If whole blood is analyzed, then use 5 μL per 100 μL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.
Please briefly centrifuge the reagent vial before use.
Use appropriate personal protective equipment per the product safety data sheet when using this product.
REFERENCES
- van Ewijk W. et al. 1981. J. Immunol.127:2594-2604.
- Ledbetter JA, 1980. J Exp Med.152:280-295.
For research use only. Not intended for use in diagnostic procedures.
cFluor® BYG750 is a tandem dye made with R-PE. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.