cFluor® BYG750 Anti-Mouse CD4 (RM4-5)
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Description
The rat monoclonal antibody clone RM4 binds to mouse CD4, a 55 kDa protein that acts as a co-receptor for the T cell receptor (TCR) in its interaction with major histocompatibility complex (MHC) class II molecules on antigen-presenting cells. The extracellular domain of CD4 binds to the beta2-domain of MHC class II, while its cytoplasmic tail provides a binding site for the tyrosine kinase Lck, facilitating the signaling cascade that initiates T cell activation. CD4 is typically expressed on thymocytes, certain mature T cell populations such as Th17 and T regulatory (Treg) cells, and on dendritic cells. If used together, the RM4-5 antibody and an alternative antibody, Anti-Mouse CD4 clone GK1.5, will "compete" for binding, i.e., the RM4-5 antibody is able to block GK1.5 antibody binding to cells. In contrast, the RM4-5 antibody does not block the binding of Anti-Mouse CD4 clone RM4-4 to cells.
PRODUCT DETAILS
Clone: RM4-5
Specificity: CD4
Reactivity: Mouse
Format: cFluor® BYG750
Isotype: Rat IgG2a, k
Tested Dilution: 5 µl/Test
Application: Flow Cytometry
Storage: 2-8°C protected from light. Do not freeze
Formulation: Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (BSA Country of Origin USA)
RECOMMENDED USAGE
Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 μL per 1 million cells in a staining volume of 100 μL. If whole blood is analyzed, then use 5 μL per 100 μL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.
Please briefly centrifuge the reagent vial before use.
Use appropriate personal protective equipment per the product safety data sheet when using this product.
For research use only. Not intended for use in diagnostic procedures.
cFluor® BYG750 is a tandem dye made with R-PE. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.