The L17F12 monoclonal antibody binds to human CD5, a 67-kDa single chain type I glycoprotein in the scavenger receptor family⁽¹⁾⁽²⁾.  CD5, also known as Leu-1, Ly-1 or T1, is expressed on thymocytes, T cells and subsets of B cells. CD5 binds to its ligand on B cells, CD72 (LYB-2), to play a role in T-B cell interaction and to modulate the intensity of antigen receptor signal transduction⁽³⁾. The antibody was conjugated to a fluorophore and purified by affinity chromatography.

PRODUCT DETAILS

Clone:         L17F12

Reactivity:    Human

Format:        cFluor^® BYG750*

Isotype:              Mouse IgG2a, κ

Tested Dilution:  5 μL / test

Application:        Flow Cytometry

Storage:               2-8°C and protected from light. Do not freeze

Formulation:       Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (Origin USA)

Data Sheets:       TDS SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

REFERENCES

1. Engleman EG, et al. 1981. Proc Natl Acad Sci USA. 78(3):1791-5
2. Velasco-de Andrés M, et al. 2020. Cells vol. 9,12 2589
3. Tabbekh M, et al. 2013. Oncoimmunology 2,1

*cFluor^® BYG750 is a tandem dye made with R-PE. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.

For Research Use Only. Not intended for use in diagnostic procedures.