cFluor® BYG710 Anti-Mouse CD49b (HMa2)
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Description
The HMa2 monoclonal antibody binds to mouse CD49b, a 150 kDa glycoprotein member of the integrin family. CD49b, also known as integrin alpha2, associates non- covalently with CD29 (integrin beta1) to form the VLA-2 heterodimer. VLA-2 integrins function as cellular receptors for collagen and laminin. CD49b is expressed by platelets, intestinal epithelial cells, NK cells, epithelial cells, and a subset of CD4+ T cells. CD49b plays an essential role in mediating the adhesion of cells to the extracellular matrix.
The antibody was conjugated to a fluorophore and purified by chromatography.
PRODUCT DETAILS
Clone: HMa2
Specificity: CD49b
Reactivity: Mouse
Format: cFluor® BYG7101
Isotype: Armenian Hamster IgG1
Tested Dilution: 5 µL / test
Application: Flow Cytometry
Storage: 2-8°C and protected from light. Do not freeze
Formulation: Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (BSA Country of Origin USA)
RECOMMENDED USAGE
Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.
Please briefly centrifuge the reagent vial before use.
Use appropriate personal protective equipment per the product safety data sheet when using this product.
REFERENCES
1. Miyake S, Sakurai T, Okumura K, Yagita H. 1994. Eur J Immunol. 24:2000-2005.
2. Noto K, Kato K, Okumura K, Yagita H. 1995. Int Immunol. 7:835-842.
3. Arase H, Saito T, Phillips JH, Lanier LL. 2001. J Immunol. 167:1141-1144.
4. Zhang Z, Ramirez NE, Yankeelov TE, Li Z, Ford LE, Qi Y, Pozzi A, Zutter MM. 2008. Blood. 111:1980-1988.
For research use only. Not intended for use in diagnostic procedures.
1cFluor® BYG710 is a tandem dye made with R-PE. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.