The BC96 monoclonal antibody binds human CD25, a 55 kDa type I transmembrane glycoprotein. CD25 is known as the low-affinity interleukin-2 receptor alpha chain (IL-2Rα). CD25 is expressed on progenitor lymphocytes, activated T & B cells, and monocytes, as well as on regulatory T cells(1-4). When CD25 is in association with CD122, IL-2 receptor beta chain and with CD132, the common gamma chain, the resulting complex is the high-affinity IL-2 receptor. IL-2 receptor signaling is key in lymphocyte proliferation and survival(5). The antibody was conjugated to a fluorophore and purified by chromatography.

PRODUCT DETAILS

Clone:          BC96

Reactivity:     Human, Non-human primate

Format:          cFluor^® BYG710¹

Isotype:              Mouse IgG1, κ

Tested Dilution:  5 μL / test

Application:        Flow Cytometry

Storage:               2-8°C and protected from Light. Do not freeze

Formulation:       Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (Origin USA)

Data Sheets:       TDS SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

REFERENCES

1. Liao W, et al. Curr Op Immunol. 23, 598 (2011)
2. Sakaguchi S, et al. J Immunol. 155, 1151 (1995)
3. Brisslert M, et al. Immunology. 117, 548 (2006)
4. Scheibenbogen C, et al. Research in Immunology. 143, 33 (1992)
5. Ross SH & Cantrell DA. Annu Rev Immunol. 36, 411 (2018)
6. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York

 ¹cFluor^® BYG710 is a tandem dye made with R-PE. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.