The rat monoclonal antibody clone RM4 binds to mouse CD4, a 55 kDa protein that acts as a co-receptor for the T cell receptor (TCR) in its interaction with major histocompatibility complex (MHC) class II molecules on antigen-presenting cells. The extracellular domain of CD4 binds to the beta2-domain of MHC class II, while its cytoplasmic tail provides a binding site for the tyrosine kinase Lck, facilitating the signaling cascade that initiates T cell activation. CD4 is typically expressed on thymocytes, certain mature T cell populations such as Th17 and T regulatory (Treg) cells, and on dendritic cells. If used together, the RM4-5 antibody and an alternative antibody, Anti-Mouse CD4 clone GK1.5, will "compete" for binding, i.e., the RM4-5 antibody is able to block GK1.5 antibody binding to cells. In contrast, the RM4-5 antibody does not block the binding of Anti-Mouse CD4 clone RM4-4 to cells.

PRODUCT DETAILS

Clone: RM4-5

Specificity: CD4

Reactivity: Mouse

Format: cFluor^® B532

Isotype: Rat IgG2a, k

Tested Dilution: 5 µl/Test

Application: Flow Cytometry

Storage: 2-8°C protected from light. Do not freeze

Formulation: Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (BSA Country of Origin USA)

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 μL per 1 million cells in a staining volume of 100 μL. If whole blood is analyzed, then use 5 μL per 100 μL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

For research use only. Not intended for use in diagnostic procedures.

cFluor^® B532 is equivalent to CF^®503R manufactured and provided by Biotium, Inc. under an Agreement between Biotium and Cytek (LICENSEE). The manufacture, use, sale, offer for sale, or import of the product is covered by one or more of the patents or pending applications owned or licensed by Biotium. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel.