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Area Scaling Factor: What It Is, and How to Use It

Learn what the Area Scaling Factor (ASF) adjustment on your flow cytometer is and how to optimize it for your cell samples. The first half of the video discusses a couple examples of ASF and how it impacts the measured area signal intensity of your cell samples. Then around minute 8, watch a demonstration of how to evaluate and adjust the ASFs within Cytek's SpectroFlo software for a cell line expressing a fluorescent protein.