PRODUCT DETAILS

Host: Rat

Isotype: IgG1, kappa

Clonality: Monoclonal

Clone: MP4-25D2

Reactivity: Hu

Application: Flow Cytometry

Tested Dilution: Assay-Dependent

Concentration: 0.5 mg/mL

Storage: 4°C

Formulation: PBS with 0.09% sodium azide; pH 7.2

Purification: Affinity chromatography

Data Sheet: TDS

Specific Information

Description: The MP4-25D2 antibody reacts with human interleukin-4 (IL-4), a 15-19 kDa cytokine secreted by Th2 cells.

Applications Reported: MP4-25D2 has been reported for use in neutralizing IL-4 binding and biological activity. (Please use Functional Grade purified MP4-25D2, Product # 16-7048 , in functional assays.) Please refer to the supporting protocols for more information.

Applications Tested: The purified MP4-25D2 can be used for blocking of fluorochrome conjugated MP4-25D2 in intracellular flow cytometry experiments to serve as specificity control. This biotinylated format for this lot of MP4-25D2 has been tested by sandwich ELISA for detection of human IL-4 in combination with 8D4-8 and can be used at 1 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

 

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.