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製品情報

Cytek Amnis CellStream ベンチトップフローサイトメーターシステムは、特許取得済みのTime Delay Integration Charge Coupled Device(TDI CCD)カメラ技術を使用した高度にカスタマイズ可能なコンパクトフローサイトメーターです。微小粒子の研究に優れた感度を提供します。直感的なソフトウェアとハイスループットな取得機能を組み合わせることで、Amnis CellStreamシステムは複雑な細胞集団を解析しながらリアルタイムで細胞を観察することができ、品質管理と一貫した結果の取得がこれまで以上に容易になります。

アップグレードが可能なCellStreamシステムを使用すると、さまざまなアプリケーションに対して再現性のあるマルチパラメータのシングルセルデータを取得できます。

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比類なき感度

最大7種の蛍光レーザーを搭載可能なCellStreamは、FITCのMESF値が<10、PEのMESF値は<5です。
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柔軟性

1〜7種のレーザーに合わせて装置を最適化でき、アップグレードは設置先で簡単に行えるため、装置のダウンタイムはありません。
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特許取得済みの光学技術

TDI CCDカメラ技術は、細胞画像を迅速にキャプチャーし、ハイスループットで光の強度データに変換します。

Designed for Seamless Integration and Improved Cytometry Workflows​​

CellStream flow cytometry systems enable walkaway sample handling for high-throughput assays. Mixing across the entire 96 well plate ensures cells stay in suspension during sampling. The sample probe is washed between samples with on-board fluidics to prevent carryover, eliminating contamination between samples.

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Cytek® Amnis® CellStream® Specifications

Parameter Performance
Fluorescence Sensitivity

MESF <10 FITC

MESF <5 PE

CV* (Precision) <3%
Number of Channels Up to 22 (20 Fluorescent, plus FSC and SSC)
Number of Lasers 1-7
Available Lasers 375, 405, 488, 532, 561, 642, 730 nm
Camera-Enabled Morphology Parameters 3 (Area; Aspect Ratio; Raw Max Pixel)
Event Rate 20,000 Cells/Sec
Flow Rates

3.66 µL/Min (Low-Speed/High-Sensitivity)

14.64 µL/Min (High-Speed)

Scatter Resolution

FSC <300 nm Using 450 nm LED

SSC <200 nm Using 785 nm Laser

Dynamic Range 7 Decades
System Size 440 mm x 625 mm x 495 mm
Field Upgradable Yes
Sample Formats Single Tube or 96-Well Plate
Absolute Cell Counting Yes
 

*Coefficient of Variation Using Chicken Erythrocyte Nuclei (CEN)

Compatible With Many Common Fluorochromes and Fluorescent Reagents

Thoughtful laser and detector selection ensures that CellStream instruments are compatible with familiar and trusted fluorophores, as well as many of the newer dyes that offer advantages, such as increased brightness and photo-stability.

High Fluorescence Sensitivity: 8-Peak Rainbow Beads
The fluorescence sensitivity of the CellStream flow cytometry platform was evaluated using industry standard 8-peak Spherotech rainbow calibration beads. The data demonstrate high fluorescence sensitivity of the CellStream system:​
  • All eight peaks are clearly resolved on every detection channel​
  • Low MESF values are determined​
  • MESF <10 FITC; MESF <5 PE

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Superior Small, Submicron Particle Detection: Size Bead Data

Size bead data demonstrates superior detection and discrimination of small particles on the CellStream platform.

The figures show acquisition of ApogeeMix containing silica spheres with diameters of 180 nm, 240 nm, 300 nm, 590 nm, 880 nm, and 1,300 nm. This mix also contains 80 nm, 110 nm, and 500 nm green fluorescent beads.

Options

The CellStream® system offers field upgradeable options for a configuration customized to your experiments.

  • Up to 7 lasers 375 nm, 405 nm, 488 nm, 532 nm, 561 nm, 642 nm and 730 nm​
  • All systems include a 488 nm laser and autosampler

CellStream
Master Bead Lot Files for Calibration
Cytek® Amnis® CellStream® CS-110107E GAN01: 06/21/2021
Cytek® Amnis® CellStream® CS-110607E-Rev2 GAL01: 10/08/2020
Cytek® Amnis® CellStream® CS-Factory-110107E GAM01: 06/11/2020
Cytek® Amnis® CellStream® Master Lot No. 110107-E Lot No. GAN02
Cytek® Amnis® CellStream® Master Lot No. 110107-E Lot No. GAN03
Cytek® Amnis® CellStream® Master Lot No. 110107-E Lot No. GAN04

For Research Use Only. Not for use in diagnostic or therapeutic procedures.