The rat monoclonal antibody clone PC61.5.3 binds to mouse CD25, a 55 kDa surface protein also known as the Interleukin-2 Receptor alpha chain or IL-2R alpha. CD25 may bind IL-2 by itself, although with low affinity and without induction of cell signaling. CD25 is also expressed within a high-affinity complex, along with the IL-2R beta chain (CD122) and the common gamma chain (CD132), to form a signaling receptor complex. The expression of CD25 varies during the developmental stages of T and B cells, is induced in activated mature T and B cells, and is present in subsets of dendritic cells. CD25 signaling, as part of the IL-2 receptor complex, triggers T cell activation and proliferation, as well as modulating the differentiation and function of Th17 cells, T regulatory (Treg) cells, and dendritic cells. The expression of CD25, CD4, and the transcription factor Foxp3 are regarded as phenotypic signatures of Treg cells.

PRODUCT DETAILS

Clone: PC61.5.3

Specificity: CD25

Reactivity: Mouse

Format: cFluor^® UV395

Isotype: Rat IgG1, l

Tested Dilution: 5 µl/Test

Application: Flow Cytometry

Storage: 2-8°C protected from light. Do not freeze

Formulation: Phosphate-buffered saline, pH 7.2, containing 0.1% PEG 3350, 0.1% Pluronic F-68, 0.05% Tween-20, 0.09% sodium azide and 1% BSA (BSA Country of Origin USA)

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 μL per 1 million cells in a staining volume of 100 μL. If whole blood is analyzed, then use 5 μL per 100 μL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

For research use only. Not intended for use in diagnostic procedures.

Cytek^® cFluor^® UV395 is StarBright^™ UltraViolet 400 Dye, manufactured and provided by Bio-Rad, Inc.

StarBright is a trademark of Bio-Rad Laboratories, Inc. in certain jurisdictions. StarBright Dyes are manufactured and provided by Bio-Rad under an Agreement between Bio-Rad supplied to Cytek, being subject to certain conditions.