cFluor® UV395 Anti-Human CD45RO (UCHL1)
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説明
The mouse monoclonal antibody clone UCHL1 binds to the human CD45 isoform known as CD45RO, a 220 kDa protein tyrosine phosphatase. CD45 is one of the most abundant hematopoietic markers expressed on all leukocytes (leukocyte common antigen, LCA). Various isoforms are generated and expressed in cell-specific patterns. With their broad cell distribution, CD45 isoforms are critical for many leukocyte functions by regulating signal transduction and cell activation associated with the T cell receptor, B cell receptor, and IL-2 receptor. Other forms of CD45, with restricted cellular expression, include CD45R (B220), CD45RA, CD45RB, and others. The UCHL1 antibody can be used to identify and quantify human CD45RO expression on thymocytes, activated memory T cells, monocytes, macrophages, and granulocytes.
PRODUCT DETAILS
Clone: UCHL1
Specificity: CD45RO
Reactivity: Human, Chimpanzee
Format: cFluor® UV395
Isotype: Mouse IgG2a, k
Tested Dilution: 5 µl/Test
Application: Flow Cytometry
Storage: 2-8°C protected from light. Do not freeze
Formulation: Phosphate-buffered saline, pH 7.2, containing 0.1% PEG 3350, 0.1% Pluronic F-68, 0.05% Tween-20, 0.09% sodium azide and 1% BSA (BSA Country of Origin USA)
RECOMMENDED USAGE
Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 μL per 1 million cells in a staining volume of 100 μL. If whole blood is analyzed, then use 5 μL per 100 μL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.
Please briefly centrifuge the reagent vial before use.
Use appropriate personal protective equipment per the product safety data sheet when using this product.
For research use only. Not intended for use in diagnostic procedures.
Cytek® cFluor® UV395 is StarBright™ UltraViolet 400 Dye, manufactured and provided by Bio-Rad, Inc.
StarBright is a trademark of Bio-Rad Laboratories, Inc. in certain jurisdictions. StarBright Dyes are manufactured and provided by Bio-Rad under an Agreement between Bio-Rad supplied to Cytek, being subject to certain conditions.