The mouse monoclonal antibody clone 2H7 binds to human CD20. CD20 is a phosphoprotein with a molecular weight of 33-37 kDa and has four transmembrane domains. It is present at all stages of B cell development, except for early pro-B cells and plasma cells, and plays a crucial role in B cell activation, proliferation, and differentiation. In some studies, CD20 is thought to act as a Ca2+ channel in the plasma membrane. It is also present on a T cell subset at low levels and some follicular dendritic cells.

PRODUCT DETAILS

Clone: 2H7

Specificity: CD20

Reactivity: Human, Baboon, Capuchin Monkey, Chimpanzee, Cynomolgus Monkey, Pigtail Monkey, Rhesus Macaque, Squirrel Monkey

Format: cFluor^® UV395

Isotype: Mouse IgG2b, k

Tested Dilution: 5 µl/Test

Application: Flow Cytometry

Storage: 2-8°C protected from light. Do not freeze

Formulation: Phosphate-buffered saline, pH 7.2, containing 0.1% PEG 3350, 0.1% Pluronic F-68, 0.05% Tween-20, 0.09% sodium azide and 1% BSA (BSA Country of Origin USA)

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 μL per 1 million cells in a staining volume of 100 μL. If whole blood is analyzed, then use 5 μL per 100 μL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

REFERENCES

1. Ledbetter et al. 1986. Hum Immunol. 15: 30-43.

For research use only. Not intended for use in diagnostic procedures.

Cytek^® cFluor^® UV395 is StarBright^™ UltraViolet 400 Dye, manufactured and provided by Bio-Rad, Inc.

StarBright is a trademark of Bio-Rad Laboratories, Inc. in certain jurisdictions. StarBright Dyes are manufactured and provided by Bio-Rad under an Agreement between Bio-Rad supplied to Cytek, being subject to certain conditions.